Table 3. Improvement of yield, quality, and biotic-abiotic stress tolerance in alfalfa through molecular breeding
Gene name |
Source |
Promoter |
Performance of transgenic alfalfa |
References |
AaEβF (E-beta-farnesene) |
Artemisia annua |
35S-CaMV |
Controlled aphid by changing behavior |
[67] |
s-cryIC (synthetic cryIC gene) |
Bacillus thuringiensis subsp. galleriae |
35S-CaMV |
Conferred Spodoptera pest insect resistance |
[68] |
ZxNHX and ZxVP1-1 (Cation/H+ antiporters and vacuolar membrane proton pump 1) |
Zygophyllum xanthoxylum |
35S-CaMV |
Produced better yield and good quality forage; enhanced tolerance to salinity, drought and phosphate deficiency |
[69] |
TPS1-TPS2(rehalose-6-phosphate |
Saccharomyces |
rd29A |
Increased biomass yield and accumulation of trehalose; enhanced tolerance to drought, freezing, salt, and heat stress |
[70] |
Mn-SOD(superoxide dismutase) |
Nicotiana plumbaginifolia |
35S-CaMV |
Improved herbage yield and winter survival |
[71] |
AVP1(Vacuolar |
Arabidopsis thaliana |
35S-CaMV |
Showed better photosynthesis efficiency; enhanced resistance to salt and drought |
[72] |
C4H (cinnamate 4-hydroxylase coumaroyl), C3H(shikimate 3-hydroxylase), F5H (ferulate 5-hydroxylase) |
Medicago truncatula, M. sativa |
PAL2 |
Significantly reduced lignin without significant impact on composition; improved forage digestibility |
[73] |
CS (citrate synthase gene) |
Pseudomonas aeruginosa |
Act2, RB7 |
Enhanced tolerance to aluminum (Al) stress |
[74] |
bar (bialaphos resistance gene) |
Streptomyces hygroscopicus. |
35S-CaMV |
Exhibited resistance to the herbicide glufosinate- ammonium [ammonium -DL-homoalanin-4-yl (methyl) phosphinate] |
[75] |
MsHsp23 (heat shock protein) |
Medicago sativa |
35S-CaMV |
Enhanced tolerance to oxidative and heat stress |
[8] |
Ibor (orange gene) |
Ipomoea batatas |
SWPA2 |
Increased carotenoid accumulation; enhanced tolerance to drought and salt stress; |
[76] |