Table 2. Details of (OPA-02 and OPA-04) RAPD primers.
|
Primer |
Sequence 5´→3´ |
MW (g/mol) |
Amount (1.0 OD) |
Molar extinct. Coefficiwnt (l/mol.cm) |
Conc. when solubilized 1ml(pmol/µl) |
Required solvent volume for conc. 100 pmol/µl |
Melting point (2ºC+ 4ºC rule) |
Melting point (G/C rule) |
|
OPA-02 |
TGC CGA GCT G |
3035 |
29.1µg/ 9.6nmol |
100683 |
9.6 |
96.0µl |
34.0 ºC |
21.1 ºC |
|
OPA-04 |
AAT CGG GCT G |
3059 |
27.0µg/ 8.8nmol |
108702 |
8.8 |
88.3µl |
32.0 ºC |
17.0 ºC |