Table 3. Primer properties of the Bt-Cry1Ac gene.
|
Primer name |
Sequence |
Base pairs |
Tm |
GC content |
|
Bt-Cry1Ac-Forward |
5’-CTCAAATCCCTGCAGTGAAGG-3’ |
21 |
55.90C |
52.4% |
|
Bt-Cry1Ac-Reverse |
5’- CATCACTGAGTCGCTTCGC-3’ |
19 |
56.20C |
57.9% |